Thiobarbituric Acid Spray Reagent for Deoxy Sugars and Sialic Acids
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چکیده
منابع مشابه
The thiobarbituric acid assay of sialic acids.
Members of the sialic acid family of compounds occur in a variety of tissues and body fluids (la, 2) and in bacteria (3), incorporated in conjugated protein. The naturally occurring sinlic acids are substituted neuraminic acid derivatives (Nacetyl, N-glycolyl, N, 0-diacetylneuraminic acid). These are collectively termed “sialic acids.” The unsubstituted 9 carbon chain is called neuraminic acid ...
متن کاملTerminology for sialic acids.
We write on behalf of the Nomenclature Committee of the International Union of Biochemistry to comment on the proposal by Scott et al. (1982) to change the name of neuraminic acid to amino-sialosonic acid as a consequence of allotting the name sialose to a hypothetical 3-deoxynonulose. They further propose symbolizing sialose as Sia, sialosonic acid as SiaA, and aminosialosonic acid as SiaNA. W...
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Use of o-phenanthroline as a reagent for determination of sialic acids was proposed by a previous investigator. This method was based on an increase in absorbance at 307 nm that occurred when solutions of o-phenanthroline and various sialic acids were mixed. It was postulated that the increased absorbance was a result of formation of specific complexes. Using N-acetylneuraminic acid, we found n...
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With dimethyl sulphoxide instead of butanol in the thiobarbituric acid assay for sialic acid, a non-fading chromophore with lambdamax. = 549 nm was produced in a homogeneous solution, allowing dilution of the test mixture in case of high colour yield. This test adapted well to studies on alkaline de-O-acetylation. Bovine and rat submaxillary mucins, and rabbit Tamm-Horsfall urinary sialoprotein...
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ژورنال
عنوان ژورنال: Nature
سال: 1960
ISSN: 0028-0836,1476-4687
DOI: 10.1038/186237a0